. Genes of proline metabolism in biotechnology of increasing wheat osmostability

Aim. To analyse effectiveness of introduced genes ornithine-δ-aminotransferase (oat) Medicago truncatula and fragments two copies first exon intron the gene prolinedehydrogenase (pdh) Arabidopsis thaliana, that form double stranded RNA suppressor wheat, in increase her productivity for actions ofosmotic stress. Methods. Determination content free L-proline (Pro); activity enzyme (OAT), proline dehydrogenase (PDG), indicators crop structure. Results. It was found wheat plants with an additional copy oat are characterized by increased OAT activity, which is not reflected Pro content. Analysis integrated elements a double-stranded pdh decrease PDG T2 generation biotechnological UK 322/17 95/17 gene, drought conditions were higher rates number compared to their original forms. Conclusions. Increased expression leads improved growth, has positive effect on plant water deficiency. Partial suppression causes performance due during drought.
 Keywords: transgenic plants, proline, ornithine-δ-aminotransferase, dehydrogenase, soil drought, grain productivity.